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Objective To find small molecules binding specifically to signal transducer and activator of transcription3 (STAT3) based on surface plasmon resonance (SPR) technology and confirm their inhibitory activities to STAT3. Methods The biomolecular interaction analysis T200 system based on SPR technology was used to couple the purified protein STAT3 to CM5 chip under the optimal pH conditions. The compounds with high binding response value were screened out from 50 candidate compounds derived from traditional Chinese medicines and the binding specificity was then confirmed. Biological experiments were performed to confirm the inhibitory effects of the screened compounds on STAT3. The binding pattern of STAT3 and the compound was fitted by molecular docking technique. Results More than 10 candidate molecules exhibited binding activities to STAT3 and kinetics assays revealed that only one candidate molecule, apigenin, showed specific binding. Western-blot analysis exhibited that apigenin inhibited the phosphorylation of STAT3 dose-dependently. Luciferase reporter gene assays demonstrated that apigenin also inhibited IL-6-induced STAT3 transcriptional activity in a dose-dependent manner. Molecular docking results showed that apigenin binds to the SH2 domain of STAT3, and interacts with key residues Glu638, Gln644, Gly656 and Lys658 by hydrogen bonds and with Tyr657 through π-π interactions. Conclusion Apigenin was a direct inhibitor of STAT3.
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Objective To assess differences between the medicine treatment level of military inpatients in military hospi-tal in 2009 and 2011 ,provide references for reasonable adjustments of health policy in future .Methods Medicine treatment da-ta from acute simple appendicitis ,coronary heart disease ,chronic bronchitis and type 2 diabetes from 16 military hospitals were excavated and analyzed by statistical methods to provide a comparative study of the medicine treatment level in 2009 and 2011 . Results 3748 cases from 16 hospitals showed the significant difference in the level of the medicine treatment in 2009 and 2011 , such as medicine cost ,medicine varieties ,primary medicine costs ,hospital daily medication cost ,and the date in 2011 is higher than 2009 .Conclusion It was showed that the medicine treatment level in four common multiple diseases in the military in 2009 and 2011 was changed .A long-term stability mechanism should be established for the evaluation of military medicine sup-port level ,which could provide strong evidence for future medicine policy adjustment .
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Objective: A prospective, observational study was undertaken to investigate the epidemiology of oral infection among the patients with advanced malignancies, and to investigate the effects of therapy strategies and risk factors on the incidence of oral infection
Methods: The patients with advanced malignancies were enrolled into the study. The incidence of oral infection with different malignant tumor groups or different treatment methods and the diagnoses of oral infection were confirmed. Demographic data on age, gender, bed rest time, nutritional status, smoking habit and the presence of oral prosthesis were also recorded
Results: Oral infection was prevalent in 46% [391/850] of all cancer patients, with the highest rate in oral and maxillofacial cancer group [67%], followed by Hematological malignancy group [58.6%] and other groups [ranging from 43.3% to 35.3%]. Oral candidiasis, oral herpes simplex, and oral mucositis were the popular infectious diseases in the patients. Chemotherapy and radiotherapy, especially combined radio- and chemotherapy, resulted in more oral infections compared with palliative care and surgery. Poor nutritional status and oral prosthesis were identified as independent risk factors associated with oral infection
Conclusion: Oral infection is prevalent among advanced cancer patients and associated with therapy methods and risk factors. More oral health care should be carried out for the patients with advanced malignant tumor
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Objective To investigate the expression of Matrix metalloprotein-9 (MMP-9) and Vascular Endothelial Growth Factor (VEGF) in breast cancer. HZ Methods SP immunohistochemistry was used to determine the expression of MMP-9 and VEGF in 96 breast cancer tissues. Results The positive rate of MMP-9 in breast cancer was 65.6 %,while the positive rate of VEGF was 71.9%. Both MMP-9 and VEGF postivity was correlated with pathological stage and lymphatic metastasis.There was a positive correlation between the expression of MMP-9 and that of VEGF in breast cancer tissues( P
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Objective To investigate the expression of metastin and its significance in benign breast disease,normal breast tissue and breast carcinoma.Methods SP immunohistochemistry was used to determine the expression of metastin in 16 benign breast disease,12 normal breast tissue and 56 breast carcinoma.Results The positive rates of metastin in benign breast disease,normal breast tissue and breast carcinoma were 68.8%,75% and 30.4%,respectively.The expression of metastin in breast carcinoma was much lower than that in benign breast disease and normal breast tissue(P
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[Objectivej To establish a gas chromatography (GC) method for the determination of the content of camphor, isoborneol and borneol in Anmo Ointment. [ Methods ] The determination was performed by programmed temperature GC, in which a 100% concentration of dimethylpolysiloxane was used as stationary liquid phase and the GC was equipped with FID detector at 300℃. [Results] The isolation and the linearity were fine with the rate of recovery of camphor 100.5% (RSD being 1.4%), isoborneol 99.1% (RSD being 1.8%) and borneol 100.8% (RSD being 1.3%). [Conclusion] This method is sensitive, rapid and accurate. It can be used to control the quality of Anmo Ointment.
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Objective To establish a gas chromatography(GC) method for the determination of camphor,isoborneol,borneol,cinnamaldehyde and eugenol in Tonglikang ointment.Methods Polyethylene glycol with 100 %of application concentration was used as stationary liquid phase.GC was equipped with FID detector at 250 ℃and programmed heating method was applied.Results The resolution and the linearity were good with the recovery rate of camphor being 96.21 %(RSD=1.03 %),isoborneol 97.34 %(RSD=0.99 %),borneol 97.07 %(RSD=1.00 %),cinnamaldehyde 96.00 %(RSD=1.37 %),and eugenol 100.99 %(RSD=1.07 %) respectively.Conclusion The method is convenient,rapid and accurate.It can be used to control the quality of Tonglikang ointment.